Glass-capillary-accessible Dynamic Microarray for Microinjection of Zebrafish Embryos
نویسندگان
چکیده
This paper describes a glass-capillary-accessible dynamic microarray for an efficient microinjection of zebrafish embryos. The microfluidic channel has an array of holes at the trapping sites and enabled a rapid alignment of eggs, direct injection, observation, and selective retrieval of embryos. Fertilized zebrafish eggs can be loaded without pumping, and genes of interest or antisence oligos are readily injectable using glass capillaries. We believe our chip will help highthroughput analysis of gene functions and the screening of zebrafish lines.
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